b cereus atcc 10987 strains  (ATCC)

Journal: Scientific Reports

Article Title: Effect of carbohydrate substrates on growth and enterotoxin gene expression in Bacillus cereus ( pacificus )

doi: 10.1038/s41598-025-31689-5

Figure Lengend Snippet: Growth curves of Bacillus cereus ATCC 10987 in the presence of various carbohydrates at different incubation temperatures. B. cereus was cultured in control medium supplemented with individual carbohydrates at 37 °C ( A ), 30 °C ( B ), 25 °C ( C ), 20 °C ( D ), and 15 °C ( E ). Carbohydrates: fructose (FRU, light blue), galactose (GAL, yellow), glucose (GLU, green), lactose (LAC, purple), sucrose (SUC, red), xylitol (XYL, dark blue), and control with no carbohydrate added (CTRL, dashed black line). The modified Gompertz model was applied to the experimental data. Statistical evaluation of the model fitting is provided in Supplement (Table S3).

Article Snippet: Although B. cereus ATCC 10987 strain was able to grow on all tested carbohydrates, the three fermentable substrates (glucose, sucrose, fructose) are among the most accessible carbohydrate energy sources in the human gastrointestinal tract .

Techniques: Incubation, Cell Culture, Control, Modification

Journal: Scientific Reports

Article Title: Effect of carbohydrate substrates on growth and enterotoxin gene expression in Bacillus cereus ( pacificus )

doi: 10.1038/s41598-025-31689-5

Figure Lengend Snippet: Transcriptomic analysis of Bacillus cereus ATCC 10987 grown on various carbohydrates at 37 °C. UpSet plots showing the number and overlap of significantly upregulated ( A ) and downregulated ( B ) genes across the carbon sources tested. Note that sucrose, glucose, and fructose affected a relatively large set of genes, showing broadly similar transcriptional responses. Besides, sucrose uniquely repressed a group of genes not downregulated by any other tested carbohydrate; to a lesser extent, lactose exhibited the same trend.

Article Snippet: Although B. cereus ATCC 10987 strain was able to grow on all tested carbohydrates, the three fermentable substrates (glucose, sucrose, fructose) are among the most accessible carbohydrate energy sources in the human gastrointestinal tract .

Techniques:

Journal: Scientific Reports

Article Title: Effect of carbohydrate substrates on growth and enterotoxin gene expression in Bacillus cereus ( pacificus )

doi: 10.1038/s41598-025-31689-5

Figure Lengend Snippet: Metabolic pathways differentially expressed in Bacillus cereus ATCC 10987 grown on various carbohydrates at 37 °C. The colour scale indicates the extent of up/downregulation, grey indicates that the enrichment score could not be calculated. Note the results of the clustering analysis above the heatmap.

Article Snippet: Although B. cereus ATCC 10987 strain was able to grow on all tested carbohydrates, the three fermentable substrates (glucose, sucrose, fructose) are among the most accessible carbohydrate energy sources in the human gastrointestinal tract .

Techniques:

Journal: Scientific Reports

Article Title: Effect of carbohydrate substrates on growth and enterotoxin gene expression in Bacillus cereus ( pacificus )

doi: 10.1038/s41598-025-31689-5

Figure Lengend Snippet: Expression of enterotoxin-encoding genes in Bacillus cereus ATCC 10987 grown on various carbohydrates at 37 °C. Statistical significance is indicated as follows: p adj ≤ 0.05 (*), p adj ≤ 0.01 (**).

Article Snippet: Although B. cereus ATCC 10987 strain was able to grow on all tested carbohydrates, the three fermentable substrates (glucose, sucrose, fructose) are among the most accessible carbohydrate energy sources in the human gastrointestinal tract .

Techniques: Expressing

Journal: Scientific Reports

Article Title: Effect of carbohydrate substrates on growth and enterotoxin gene expression in Bacillus cereus ( pacificus )

doi: 10.1038/s41598-025-31689-5

Figure Lengend Snippet: Growth curves of Bacillus cereus ATCC 10987 in the presence of various carbohydrates at different incubation temperatures. B. cereus was cultured in control medium supplemented with individual carbohydrates at 37 °C ( A ), 30 °C ( B ), 25 °C ( C ), 20 °C ( D ), and 15 °C ( E ). Carbohydrates: fructose (FRU, light blue), galactose (GAL, yellow), glucose (GLU, green), lactose (LAC, purple), sucrose (SUC, red), xylitol (XYL, dark blue), and control with no carbohydrate added (CTRL, dashed black line). The modified Gompertz model was applied to the experimental data. Statistical evaluation of the model fitting is provided in Supplement (Table S3).

Article Snippet: On the other hand, our study provides a comprehensive analysis of how different carbohydrates and incubation temperatures affect B. cereus ATCC 10987 strain growth and the regulation of key metabolic pathways, including the expression of enterotoxin genes.

Techniques: Incubation, Cell Culture, Control, Modification

Journal: Scientific Reports

Article Title: Effect of carbohydrate substrates on growth and enterotoxin gene expression in Bacillus cereus ( pacificus )

doi: 10.1038/s41598-025-31689-5

Figure Lengend Snippet: Transcriptomic analysis of Bacillus cereus ATCC 10987 grown on various carbohydrates at 37 °C. UpSet plots showing the number and overlap of significantly upregulated ( A ) and downregulated ( B ) genes across the carbon sources tested. Note that sucrose, glucose, and fructose affected a relatively large set of genes, showing broadly similar transcriptional responses. Besides, sucrose uniquely repressed a group of genes not downregulated by any other tested carbohydrate; to a lesser extent, lactose exhibited the same trend.

Article Snippet: On the other hand, our study provides a comprehensive analysis of how different carbohydrates and incubation temperatures affect B. cereus ATCC 10987 strain growth and the regulation of key metabolic pathways, including the expression of enterotoxin genes.

Techniques:

Journal: Scientific Reports

Article Title: Effect of carbohydrate substrates on growth and enterotoxin gene expression in Bacillus cereus ( pacificus )

doi: 10.1038/s41598-025-31689-5

Figure Lengend Snippet: Metabolic pathways differentially expressed in Bacillus cereus ATCC 10987 grown on various carbohydrates at 37 °C. The colour scale indicates the extent of up/downregulation, grey indicates that the enrichment score could not be calculated. Note the results of the clustering analysis above the heatmap.

Article Snippet: On the other hand, our study provides a comprehensive analysis of how different carbohydrates and incubation temperatures affect B. cereus ATCC 10987 strain growth and the regulation of key metabolic pathways, including the expression of enterotoxin genes.

Techniques:

Journal: Scientific Reports

Article Title: Effect of carbohydrate substrates on growth and enterotoxin gene expression in Bacillus cereus ( pacificus )

doi: 10.1038/s41598-025-31689-5

Figure Lengend Snippet: Expression of enterotoxin-encoding genes in Bacillus cereus ATCC 10987 grown on various carbohydrates at 37 °C. Statistical significance is indicated as follows: p adj ≤ 0.05 (*), p adj ≤ 0.01 (**).

Article Snippet: On the other hand, our study provides a comprehensive analysis of how different carbohydrates and incubation temperatures affect B. cereus ATCC 10987 strain growth and the regulation of key metabolic pathways, including the expression of enterotoxin genes.

Techniques: Expressing